This data set contains soil and xylem water delta18O and delta2H values of soil and plant tissue samples collected in different land use systems around Santa Fe, Central Panama in 2008. The samples were collected and analysed by Francois Jost for his Masterthesis entitled "Using Stable Isotopes (D, 18O) to Estimate Plant Water Uptake in Different Vegetation Types, Panama", University of Goettingen, 2009 under the supervision of Dr. Luitgard Schwendenmann.