data test

We report here our mechanistic study of the previously published nickel-catalyzed cyclopropanation reaction using lithiomethyltrimethylammonium triflate as methylene donor. The cyclopropane yield is highly dependent on the olefin substrate and correlates well with the binding affinity of the olefin to Ni(0) as established elsewhere. On the basis of this...

Coefficients of the model fitted to the polyketide dose–BY4741 growth response curves.

Coefficients of the model fitted to the polyketide dose–Sf9 cell survival curves.

X-linked juvenile retinoschisis (XLRS) is a hereditary retinal dystrophy, caused by mutations in the RS1 gene which encodes the secreted protein retinoschisin. In recent years, several molecules have been proposed to interact with retinoschisin, including the retinal Na/K-ATPase, L-voltage gated Ca2+ channels, and specific sugars. We recently showed that...

Mean fluorescence intensity (MFI) from total cell population, calibrated against non-mutant ATP1B2. Given is the mean +/- SD of 3 (patch II region 4 T240A and S) or 4 (other patch II mutants) independent experiments. Neg. control: untransfected cells.

Amino acid (aa) positions and sequences of the each four regions building the predicted protein-protein-interfaces patch I and patch II of ATP1B2 as well as corresponding aa sequences in ATP1B1.

Mean fluorescence intensity (MFI) from total cell population, calibrated against non-mutant ATP1B2. Given is the mean +/- SD of 3 independent experiments. Neg. control: untransfected cells.

Method validation parameters of NAAB-503 and NAAB-496.

Selectivity index (SI) and cytotoxic and antiviral activity of NAAB-496 and NAAB-503 against HSV-1.

FindingsHere, we demonstrate that OP2113 (5-(4-Methoxyphenyl)-3H-1,2-dithiole-3-thione, CAS 532-11-6), synthesized and used as a drug since 1696, does not act as an unspecific antioxidant molecule (i.e., as a radical scavenger) but unexpectedly decreases mitochondrial reactive oxygen species (ROS/H2O2) production by acting as a specific inhibitor of ROS...

Complementation of epa transposon mutants.

Enterococcus faecalis is an opportunistic pathogen with an intrinsically high resistance to lysozyme, a key effector of the innate immune system. This high level of resistance requires a complex network of transcriptional regulators and several genes (oatA, pgdA, dltA and sigV) acting synergistically to inhibit both the enzymatic and cationic...

MIC of lysozyme for Firmicutes.

Although alkynes have been extensively exploited in [2 + 2 + 2] cycloadditions with nitriles to form N-heterocyclic aromatics, the “alkyne-like” metal–carbon triple bond has never been used in [2 + 2 + 2] cycloadditions with nitriles. We demonstrate the synthesis of the first metallapyrazine through [2 + 2 + 2] cycloaddition reactions of a metal–carbyne...

Multicomponent polymerizations (MCPs) as a burgeoning field in polymer chemistry has proved to be a powerful and popular tool for the synthesis of functional polymer materials with diverse and complex structures. To explore the general applicability of MCPs and enrich the product structures of MCPs, multicomponent tandem polymerizations (MCTPs) with great...

Multicomponent polymerizations (MCPs) as a burgeoning field in polymer chemistry has proved to be a powerful and popular tool for the synthesis of functional polymer materials with diverse and complex structures. To explore the general applicability of MCPs and enrich the product structures of MCPs, multicomponent tandem polymerizations (MCTPs) with great...

The synthesis and characterization of an analog of the Xantphos ligand that is geometrically incapable of coordination of the xanthene bridging oxygen atom is reported. This new ligand, XL-Xantphos, ((9,9-dimethyl-9H-xanthene-4,5-diyl)­bis­(4,1-phenylene))­bis­(diphenylphosphane), was studied in homogeneous, catalytic reactions for comparison with...

Sensitivity and specificity analysis of the multiplex assay compared to the commercial ELISA-based CCP3 test, regarding peptide biomarker selection.

Performance of the eight citrullinated peptides studied and their comparison with the commercial ELISA-based CCP3 test.

Modular self-assembly of biomolecules in two dimensions (2D) is straightforward with DNA but has been difficult to realize with proteins, due to the lack of modular specificity similar to Watson–Crick base pairing. Here we describe a general approach to design 2D arrays using de novo designed pseudosymmetric protein building blocks. A homodimeric helical...